Cancer Cell (2006) 9:405-416. Published: 2006.05.15
Richard Smith, Leah A. Owen, Deborah J. Trem, Jenny S. Wong, Jennifer S. Whangbo, Todd R. Golub, and Stephen L. Lessnick
Read ManuscriptOur understanding of Ewing's sarcoma development mediated by the EWS/FLI fusion protein has been limited by a lack of knowledge regarding the tumor cell of origin. To circumvent this, we analyzed the function of EWS/FLI in Ewing's sarcoma itself. By combining retroviral-mediated RNA interference with reexpression studies, we show that ongoing EWS/FLI expression is required for the tumorigenic phenotype of Ewing's sarcoma. We used this system to define the full complement of EWS/FLI-regulated genes in Ewing's sarcoma. Functional analysis revealed that NKX2.2 is an EWS/FLI-regulated gene that is necessary for oncogenic transformation in this tumor. Thus, we developed a highly validated transcriptional profile for the EWS/FLI fusion protein and identified a critical target gene in Ewing's sarcoma development.
| Description | Link/Filename |
|---|---|
| Manuscript | Smith et al.pdf |
| Preview article | Cancer Cell preview.pdf |
| CEL file descriptions | Smith_et_al_CEL_file_list.xls |
| CEL files for knockdown samples | Smith_etal_knockdown_CEL_files.zip |
| CEL files for inducible samples | Smith_etal_inducible_CEL_files.zip |
| Supplemental appendix 1 | Smith, et al., supplemental appendix 1.doc |
| Supplemental appendix 2 | Smith, et al., supplemental appendix 2.xls |
| Supplemental appendix 3 | Smith, et al., supplemental appendix 3.xls |