A Strategy for Oligonucleotide Microarray Probe Reduction

Genome Biology 2002, 3(12):research0073.1?0073.4. Published: 2002.11.24

Alena A. Antipova, Pablo Tamayo, and Todd R. Golub

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Abstract

Background:
One of the factors limiting the number of genes analyzable on high density oligonucleotide arrays is that each transcript is probed by multiple oligonucleotide probes of distinct sequence in order to magnify the sensitivity and specificity of detection. Over the years, the number of probes per gene has decreased, but still no single array for the entire human genome has been reported. To reduce the number of probes required for each gene, a robust systematic approach for choosing the most representative probes is needed. Here, we introduce a generalizable empiric method for reducing the number of probes per gene while maximizing the fidelity to the original array design.

Results:
The methodology has been tested on a dataset comprised of 317 Affymetrix HuGeneFL GeneChips. The performance of the original and reduced probe sets was compared in four cancer classification problems. The results of these comparisons demonstrate that the reduction of the probe set by 95% does not dramatically affect performance, and thus illustrate the feasibility of substantially reducing probe numbers without significantly compromising sensitivity and specificity of detection.

Conclusions:
The strategy described here is potentially useful for designing small, limited-probe genome-wide arrays for screening applications.

Keywords: Average Difference probe pair probe selection

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Supplemental Data

Description Link/Filename
Description of these files AboutTheseFiles.doc
Paper in pdf format Antipova_et_al_2002.pdf
Raw feature data for all the genes on the chips RawFeatureData.tar.gz
Unscaled Delta(h), random Deltas, and Average Difference UnscaledResFiles.tar.gz
Scaled Delta(h), random Deltas, and Average Difference ScaledResFiles.tar.gz
Cls files, idealized expression vectors for class assignments ClsFiles.tar.gz
Expanded Figure 2 Fig2Features.xls
Expanded Table 1, includes classification parameters Table1Features.xls
List of selected Delta(h) probes ListOfDeltaHprobes.xls