Cell 114: 323-334 2003. Published: 2003.08.07
Justin Lamb, Sridhar Ramaswamy, Heide L. Ford, Bernardo Contreras, Robert V. Martinez, Frances S. Kittrell, Cynthia A. Zahnow, Nick Patterson, Todd R. Golub and Mark E. Ewen
Here we describe how patterns of gene expression in human tumors have been deconvoluted to reveal a previously unappreciated mechanism of action for the cyclin D1 oncogene. Computational analysis of the expression patterns of thousands of genes across hundreds of tumor specimens suggested that a transcription factor, C/EBPβ/Nf-Il6, participates in the consequences of cyclin D1 overexpression. Functional analyses confirmed the involvement of C/EBPβ in the regulation of genes affected by cyclin D1 and established this protein as an indispensable effector of a potentially important facet of cyclin D1 biology. This work demonstrates that tumor gene expression databases can be used to study the function of a human oncogene in situ.
| Description | Link/Filename |
|---|---|
| full text | http://download.cell.com/pdfs/0092-8674/PIIS0092867403005701.pdf |
| Supplementary Information 1 (cyclin D1 nearest neighbors) | SI1.txt |
| Preview in Cell | http://download.cell.com/pdfs/0092-8674/PIIS0092867403006019.pdf |
| Supplementary Information 2 (GCM KSS) | SI2.txt |
| Supplementary Information 3 (prostate KSS) | SI3.txt |
| Nature News and Views | http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v424/n6951/full/424898a_fs.html&content_filetype=PDF |
| Nature Reviews in Cancer Highlights | http://www.nature.com/cgi-taf/DynaPage.taf?file=/nrc/journal/v3/n9/full/nrc1175_fs.html&filetype=pdf |
| Supplementary Information 4 (lung KSS) | SI4.txt |
| Supplementary Information 5 (brain KSS) | SI5.txt |
| Nature Genetics Research Notes | http://www.nature.com/cgi-taf/DynaPage.taf?file=/ng/journal/v35/n1/full/ng0903-19.html&filetype=PDF |
| Supplementary Information 6 (NCI60 KSS) | SI6.txt |
| Supplementary Information 7 (chromosome coordinates) | SI7.pdf |
| Supplementary Information 8 (competitor oligonucleotides) | SI8.pdf |
| Supplementary Information 9 (additional panels for Figure 6D) | SI9.pdf |
| Supplementary Information 10 (additional panels for Figure 6E) | SI10.pdf |
| raw gene expression data | raw_data.res |