Construct: shRNA TRCN0000072219

Construct Description:

Construct Type:
shRNA
Other Identifiers:
rfp_576s1c1
DNA Barcode:
CTACAAGACCGACATCAAGCT

Original Target:

Taxon:
Control
Gene:
RFP (RFP)
Gene Description:
RFP
Transcript:
Broad rfp.1 (CURRENT)
Match location:
Position 576 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000072219)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL RFP RFP RFP rfp.1 100% CDS 576 2.640 N/A
2 human 90665 TBL1Y transducin (beta)-like 1, Y... XM_017030087.1 85% 3UTR 2404 N/A N/A
Download CSV

Sequence Information

Target Sequence:
CTACAAGACCGACATCAAGCT
Hairpin Sequence:
5'-CCGG-CTACAAGACCGACATCAAGCT-CTCGAG-AGCTTGATGTCGGTCTTGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACAAGACCGACATCAAGCTCTCGAGAGCTTGATGTCGGTCTTGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACAAGACCGACATCAAGCTCTCGAGAGCTTGATGTCGGTCTTGTAG-3'

Other clones with same target sequence:

  1. TRCN0000231689