Construct: shRNA TRCN0000231717

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_27s21c1
DNA Barcode:
CGTCGTATTACAACGTCGTGA

Original Target:

Taxon:
Control
Gene:
lacZ (lacZ)
Gene Description:
lacZ
Transcript:
Broad lacZ.1 (CURRENT)
Match location:
Position 27 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231717)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCGTATTACAACGTCGTGA
Hairpin Sequence:
5'-CCGG-CGTCGTATTACAACGTCGTGA-CTCGAG-TCACGACGTTGTAATACGACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCGTATTACAACGTCGTGACTCGAGTCACGACGTTGTAATACGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCGTATTACAACGTCGTGACTCGAGTCACGACGTTGTAATACGACG-3'

Other clones with same target sequence:

  1. TRCN0000072232