Construct: shRNA TRCN0000231740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
promegaLuc_162s21c1
DNA Barcode:
TGAGTACTTCGAAATGTCCGT

Original Target:

Original Target:

Taxon:
Control
Gene:
LUCIFERASE (LUCIFERASE)
Gene Description:
promegaLuc
Transcript:
Broad promegaLuc.1 (CURRENT)
Match location:
Position 162 (CDS)

Vector Information:

Vector Backbone:
pLKO_TRC005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL LUCIFERASE LUCIFERASE promegaLuc promegaLuc.1 100% CDS 162 0.660 N/A
2 CONTROL Luciferase Luciferase Hahn Lab Luciferase Luciferase.1 100% CDS 162 0.660 N/A
3 human 101929657 LOC101929657 uncharacterized LOC101929657 NR_110306.1 89% 3UTR 2000 N/A N/A
4 mouse 102637449 Gm34254 predicted gene, 34254 XR_389502.2 85% 3UTR 1300 N/A N/A
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Sequence Information

Target Sequence:
TGAGTACTTCGAAATGTCCGT
Hairpin Sequence:
5'-CCGG-TGAGTACTTCGAAATGTCCGT-CTCGAG-ACGGACATTTCGAAGTACTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGTACTTCGAAATGTCCGTCTCGAGACGGACATTTCGAAGTACTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGTACTTCGAAATGTCCGTCTCGAGACGGACATTTCGAAGTACTCA-3'

Other clones with same target sequence:

  1. TRCN0000072257