Transcript: Human NM_001252007.1

Homo sapiens poly(A) polymerase alpha (PAPOLA), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
PAPOLA (10914)
Length:
4847
CDS:
218..934

Additional Resources:

NCBI RefSeq record:
NM_001252007.1
NBCI Gene record:
PAPOLA (10914)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001252007.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000053021 GCGTACTTACACAGAAACTAA pLKO.1 324 CDS 100% 5.625 3.938 N PAPOLA n/a
2 TRCN0000286909 GCGTACTTACACAGAAACTAA pLKO_005 324 CDS 100% 5.625 3.938 N PAPOLA n/a
3 TRCN0000175526 CTATTGAAACAGCCTGAAGAA pLKO.1 3748 3UTR 100% 4.950 3.465 N Papola n/a
4 TRCN0000279185 CTATTGAAACAGCCTGAAGAA pLKO_005 3748 3UTR 100% 4.950 3.465 N Papola n/a
5 TRCN0000053019 GCTATCAAACTATGGGCCAAA pLKO.1 1637 3UTR 100% 4.050 2.835 N PAPOLA n/a
6 TRCN0000053022 GCTGTTGAAGAGGCATTCGTA pLKO.1 659 CDS 100% 3.000 2.100 N PAPOLA n/a
7 TRCN0000311748 GCTGTTGAAGAGGCATTCGTA pLKO_005 659 CDS 100% 3.000 2.100 N PAPOLA n/a
8 TRCN0000279187 TTGCAGGGTAACCGATGAAAT pLKO_005 1570 3UTR 100% 13.200 7.920 N Papola n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001252007.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11570 pDONR223 100% 78% 73.8% None (many diffs) n/a
2 TRCN0000479225 GTACAGGTACTCAAGTAGGGTTCA pLX_317 64.1% 78% 73.8% V5 (many diffs) n/a
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