Transcript: Human XM_006722800.2

PREDICTED: Homo sapiens mitogen-activated protein kinase kinase 7 (MAP2K7), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAP2K7 (5609)
Length:
3536
CDS:
161..1489

Additional Resources:

NCBI RefSeq record:
XM_006722800.2
NBCI Gene record:
MAP2K7 (5609)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149491 CATTCTGGGCAAGATGACAG pXPR_003 TGG 715 54% 7 0.6646 MAP2K7 MAP2K7 77822
2 BRDN0001149362 ACGGGCTACCTGACCATCGG pXPR_003 GGG 371 28% 4 0.3137 MAP2K7 MAP2K7 77820
3 BRDN0001162319 CAAAGCACTGCACGATGTAG pXPR_003 GGG 581 44% 6 0.2579 MAP2K7 MAP2K7 77821
4 BRDN0001162371 CTTAACGGCAATGACGTGGC pXPR_003 CGG 478 36% 5 -0.1532 MAP2K7 MAP2K7 77823
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006722800.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380835 CGTCATTGCCGTTAAGCAAAT pLKO_005 640 CDS 100% 10.800 15.120 N MAP2K7 n/a
2 TRCN0000321014 GTCAAAGACTGCCTTACTAAA pLKO_005 1298 CDS 100% 13.200 9.240 N Map2k7 n/a
3 TRCN0000350455 GTCAAAGACTGCCTTACTAAA pLKO_005 1298 CDS 100% 13.200 9.240 N MAP2K7 n/a
4 TRCN0000315338 AGAACTGCAAGACGGACTTTG pLKO_005 1197 CDS 100% 10.800 7.560 N MAP2K7 n/a
5 TRCN0000360372 AGAACTGCAAGACGGACTTTG pLKO_005 1197 CDS 100% 10.800 7.560 N Map2k7 n/a
6 TRCN0000315390 GATCAGAGTCGCTGTTCATTC pLKO_005 1742 3UTR 100% 10.800 7.560 N MAP2K7 n/a
7 TRCN0000001079 CACAGGAAGAGACCAAAGTAT pLKO.1 1322 CDS 100% 5.625 3.938 N MAP2K7 n/a
8 TRCN0000196556 GCAAATCAAGTGGACAAGAAA pLKO.1 2786 3UTR 100% 5.625 3.938 N MAP2K7 n/a
9 TRCN0000195695 CAAGATGACAGTGGCGATTGT pLKO.1 868 CDS 100% 4.950 3.465 N MAP2K7 n/a
10 TRCN0000010586 CCCTACATCGTGCAGTGCTTT pLKO.1 737 CDS 100% 4.950 3.465 N MAP2K7 n/a
11 TRCN0000010588 GATTGTGAAGGCGCTGTACTA pLKO.1 883 CDS 100% 4.950 3.465 N MAP2K7 n/a
12 TRCN0000350516 GATTGTGAAGGCGCTGTACTA pLKO_005 883 CDS 100% 4.950 3.465 N MAP2K7 n/a
13 TRCN0000380424 CAGACACTGTGAACGGAAGAC pLKO_005 1711 3UTR 100% 4.050 2.835 N MAP2K7 n/a
14 TRCN0000010587 GAGCATTGAGATTGACCAGAA pLKO.1 472 CDS 100% 4.050 2.835 N MAP2K7 n/a
15 TRCN0000315389 GAGCATTGAGATTGACCAGAA pLKO_005 472 CDS 100% 4.050 2.835 N MAP2K7 n/a
16 TRCN0000001080 CTACAAGAACTGCAAGACGGA pLKO.1 1192 CDS 100% 0.660 0.462 N MAP2K7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006722800.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14811 pDONR223 0% 96.2% 96.1% None 51G>A;125_172del;621C>T n/a
2 ccsbBroad304_14811 pLX_304 0% 96.2% 96.1% V5 51G>A;125_172del;621C>T n/a
3 TRCN0000479770 GCAAAGCTAGGTTCCCCTATATCG pLX_317 26% 96.1% 95.9% V5 (many diffs) n/a
4 TRCN0000488353 GCTTAAACCCCCTGTGTGAACCAC pLX_317 26% 96.2% 96.1% V5 (not translated due to prior stop codon) 51G>A;125_172del;621C>T n/a
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