Transcript: Human XM_017006219.1

PREDICTED: Homo sapiens EPH receptor A6 (EPHA6), transcript variant X13, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
EPHA6 (285220)
Length:
1673
CDS:
44..1657

Additional Resources:

NCBI RefSeq record:
XM_017006219.1
NBCI Gene record:
EPHA6 (285220)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146458 TGGTACATGCCATAGAAGGT pXPR_003 GGG 1253 78% 4 0.9562 EPHA6 EPHA6 76247
2 BRDN0001145443 GAAAACATATCCATTAAATG pXPR_003 GGG 445 28% 2 -0.0872 EPHA6 EPHA6 76248
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017006219.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000021418 CGTGAATTACACCTTTGAAAT pLKO.1 1549 CDS 100% 13.200 18.480 N EPHA6 n/a
2 TRCN0000021415 CCCAAGCCATTCACAGCTATT pLKO.1 1607 CDS 100% 10.800 7.560 N EPHA6 n/a
3 TRCN0000021416 GCCATCACTGAAATGGATGAA pLKO.1 500 CDS 100% 4.950 3.465 N EPHA6 n/a
4 TRCN0000021417 CCTCAAACTCAACACTGAAAT pLKO.1 823 CDS 100% 13.200 7.920 N EPHA6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017006219.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15300 pDONR223 0% 47.3% 47.3% None 1607_1609delGAA;1611_1612ins1782 n/a
2 ccsbBroad304_15300 pLX_304 0% 47.3% 47.3% V5 1607_1609delGAA;1611_1612ins1782 n/a
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