Loading
NEW (2024-09-24)!For the GRCh38 genome, CRISPick has added support for gnomAD variation filtering. This feature is enabled by default, and deprioritizes selection of sgRNAs that overlap sites of variation, e.g. SNPs and indels. See the "Advanced Options" section below for details.

Reference Genome

Mechanism

Enzyme

Target(s)

Drop file here or
  • Enter up to 500 target IDs.
  • Any sequences submitted via upload must be in FASTA format and must be at least 30 bases.
  • 20KB is the max file upload size.
Accepted target formats
ID
Gene Symbol
CDC5L, Brca1
Gene ID
988
Transcript ID
NM_014911, NM_014911.1
Sequence
Raw
TTGTAGCATCGCAGGTAGCAAACAGTTACTAGG
FASTA
>seq0
TTGTAGCATCGCAGGTAGCAAACAGTTACTAGG
Coordinates
Point
NC_000001.11:+:127140001
Range
NC_000001.11:-:15000-16000
Ranges
NC_000001.11:-:12000-13000;15000-16000

CRISPick Quota

This tool will recommend the top N candidates according to:
  • Raw ranking
  • Cut position
  • Mutual spacing