Design sgRNAs Using Rule Set 1 Scores (Decommissioned 10/6/2016)

Note: Because this tool no longer supports Ensembl transcripts (due to a change in Ensembl infrastructure) and implements the outdated Rule Set 1 sgRNA scoring algorithm described in Doench, Hartenian, et al., Nature Biotechnology, 2014, it has been decommissioned.

Our current sgRNA design tool utilizes Rule Set 2 scoring and supports DNA sequence, RefSeq transcript ID, and NCBI gene ID/symbol inputs.

If you still require Rule Set 1 scores for a set of sgRNAs, there exists a stand-alone Python script, found here. Run as: python on_target_score_calculator.py <30mer>