Platform Overview

GPP develops technologies for perturbing genes and assists collaborators in experimental planning and execution by helping choose the best model system and experimental readout to assess the effects of genetic perturbation. Technologies include libraries of short hairpin RNAs (shRNAs), CRISPR/Cas9 constructs, and open reading frames (ORFs) to knock down or overexpress genes, in addition to other techniques such as TALEN and CRISPR/Cas9 for genome editing and "tough decoy" constructs to inhibit microRNAs.

Search

Search by Gene
Search by Clone

Design Tools

CRISPick (GPP sgRNA Designer)
Design sgRNA sequences for CRISPRko/a/i targets
Design Hairpins for a Target Transcript Sequence (RNAi)
Generate matching shRNA designs for a transcript sequence

Informational Resources and Downloads

Protocols
Protocols including clone, DNA, and virus production
Library Resources
Clone inventory downloads
Pooled Screening File Downloads
For file downloads for analysis of pooled screens, see our Clone Pool section.

Analysis Tools

Pooled Screen Analysis Guide
Guidance on reading PoolQ files, assessing screen quality and hit calling
Pooled Screening File Downloads
For file downloads for analysis of pooled screens, see our Clone Pool section.
Pooled Screen Analysis Tool
Hypergeometric or Negative Binomial Analysis
Software Tool Downloads
Software tools and related resources for screen analysis

Reagents

Available Libraries
Description of available clone pools with provided analysis downloads
Vectors
Description of vectors, maps and sequences

Miscellaneous

Frequently Asked Questions