GPP Web Portal - Welcome

The Genetic Perturbation Platform, formerly known as the RNA interference (RNAi) Platform, supports functional investigations of the mammalian genome that can reveal how genetic alterations lead to changes in phenotype.

GPP develops technologies for perturbing genes and assists collaborators in experimental planning and execution by helping choose the best model system and experimental readout to assess the effects of genetic perturbation. Technologies include libraries of short hairpin RNAs (shRNAs), CRISPR/Cas9 constructs, and open reading frames (ORFs) to knock down or overexpress genes, in addition to other techniques such as TALEN and CRISPR/Cas9 for genome editing and "tough decoy" constructs to inhibit microRNAs.


Search by Gene
Search by Clone


Design sgRNAs for CRISPR knockout (CRISPRko)
Generate matching sgRNA knockout (CRISPRko) designs for a mouse or human gene, transcript or target sequence
Design sgRNAs for CRISPR activation or inhibition (CRISPRa/CRISPRi)
Generate matching sgRNA activation or inhibition (CRISPRa/CRISPRi) designs for a mouse or human gene
Design Hairpins for a Target Transcript Sequence (RNAi)
Generate matching shRNA designs for a transcript sequence


Protocols including clone, DNA, and virus production
Description of vectors, maps and sequences


Software Tool Downloads
Software tools and related resources for screen analysis
Pooled Screening File Downloads
File downloads for analysis of pooled screens


Library Resources
Clone inventory downloads
Frequently Asked Questions