Construct: ORF BRDN0000395088

Failed QC, DO NOT USE

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008669.1_s300c1
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DOCK8 (81704)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

No results found.

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
6066
ORF length:
5997
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GACACACCTG AACAGCCTGG AGGGGCGgct tgcccaggag ctcggggact
121tcactgatga cgacttggac gtggtgttca cgccaaagga atgtaggact ttgcagccct
181ctttgccgga ggaaggggtt gaactggacc ctcatgtcag ggactgtgtt cagacctaca
241tccgtgagtg gctaatcgtg aaccggaaaa accaaggaag tccagaaatc tgtggcttta
301aaaagactgg atctcgaaaa gattttcaca agacgcttcc gaaacagacg tttgagtcgg
361aaaccttgga gtgcagtgaa cccgctgctc aggcaggccc ccgccactta aacgtgctgt
421gcgacgtgtc tgggaaaggc cccgtcactg cctgtgactt tgacctccgc agcctgcagc
481ctgacaagcg gctagaaaac ctcctgcagc aagtgagtgc cgaggacttt gagaagcaga
541acgaggaggc ccggaggacc aataggcagg ccgagctctt tgccctttac ccatcagtgg
601acgaggagga tgctgtggaa atacgtccag taccagaatg tcccaaggaa cacctgggca
661acagaatatt ggtcaagttg ctgaccttga agttcgagat tgaaattgag cccctgtttg
721ccagcattgc cctctacgat gttaaagaaa ggaaaaagat ctcagaaaat tttcactgtg
781acctgaactc tgaccagttc aaaggatttc tgcgagctca cacgccttca gtggccgcat
841caagtcaggc gagatctgca gtcttctcag tcacctaccc gtcctcagac atctacctgg
901tagtcaagat tgaaaaagtc ctgcagcagg gagagattgg agactgtgca gagccctaca
961cggttatcaa agaaagtgat ggtggaaaga gtaaagaaaa gattgaaaaa ctaaaactcc
1021aagctgaatc cttctgccag cgtttgggga aataccggat gccctttgcc tgggcaccca
1081taagcttatc aagcttcttc agtgtctcca cccttgagag ggaggtaact gatgtggact
1141ctgtggttgg gagaagctca gtgggtgaac ggaggacatt ggcccaatct agaaggcttt
1201ctgaaagagc cctctccttg gaggaaaatg gggttggatc caacttcaaa acctccactc
1261tgagcgttag catctttttc aagcaggaag gagatcgcct tagcgatgaa gacttattca
1321agtttttagc tgactacaaa agatcatcat ccttacagag acgagtcaag tcaattccag
1381gcttgctaag actggagatt tctacagctc cagagatcat caattgctgt ctgactcctg
1441aaatgctgcc cgtgaaaccc tttcctgaaa accggacacg cccgcacaaa gagattttgg
1501aatttccaac acgagaagta tatgtccctc acactgtgta cagaaacctt ctctatgtct
1561acccacagag gctgaacttt gtaaacaaac tagcatcagc ccggaacatt acaataaaga
1621tccagtttat gtgtggagaa gatgctagca atgcgatgcc ggtcatcttt ggaaagtcca
1681gcgggcctga atttctgcag gaagtgtaca cagctgttac ataccataat aagtctcctg
1741acttttatga agaagtgaaa attaagctcc ccgctaagct cacagtaaat caccacctcc
1801tgttcacctt ctaccatatc agctgtcagc agaagcaagg agcctccgtg gaaactctcc
1861tgggatattc atggctgcca attctcttaa atgaacgtct tcaaactgga tcctactgtc
1921tcccagttgc cttggaaaaa ttgccaccca actactccat gcattctgct gagaaagtcc
1981cattacagaa tcctcccatt aagtgggctg aaggacataa gggagtattt aatattgaag
2041tgcaagctgt ttcttctgta cacacccagg acaaccacct ggagaagttc ttcaccctct
2101gccactccct ggagagccag gtgaccttcc ccatccgcgt gctggatcag aaaatcagcg
2161agatggcgct ggagcatgag ctgaagctca gcatcatctg cctcaactcc tcccgcctgg
2221agccgctcgt gctcttcctg cacctggtgc tggacaagct cttccagctg tccgtgcagc
2281ccatggtcat cgctggccag acagccaact tctcccagtt tgccttcgag tccgtggtgg
2341ccatcgccaa cagtctgcac aacagcaagg acctgagcaa ggaccagcat gggaggaact
2401gcctgctggc ttcctacgtg cactacgtct tccgcctgcc agaggtgcaa agggatgtgc
2461ccaagtcagg cgctcccact gccctcctag accctcggag ctaccacacg tatggccgca
2521catcagctgc tgctgtgagt tcaaagctgc tgcaggcccg ggtgatgagc agcagtaacc
2581cagacctcgc ggggacacac tccgcagcag acgaggaagt gaagaacatc atgtcttcaa
2641agcatttcca tgaggagctt gcccttcaga tggtggtcag cactggaatg gtgagagaaa
2701cagtcttcaa gtatgcctgg ttcttctttg agcttctggt gaaaagcatg gcccagcacg
2761tacataacat ggacaaacgg gacagttttc ggaggactcg tttttctgac cgtttcatgg
2821atgacataac tactattgtt aatgtggtca cctcggaaat tgcagccctt ttagtaaaac
2881cacagaagga aaatgaacag gcggaaaaga tgaacatcag cctggctttc ttcttgtatg
2941accttctctc cctcatggat cggggctttg tgtttaacct catcagacat tattgcagcc
3001agctgtcagc caagctcagt aaccttccaa cgctcatttc catgaggcta gagttcctga
3061gaatcctctg tagccatgag cattacctca atctgaacct tttttttatg aatgctgata
3121ctgctccaac atctccttgt ccttccatat cttcccagaa ctcaagctcc tgctccagct
3181tccaggacca gaagatcgcc agcatgttcg atctgacttc cgagtaccgc cagcagcact
3241tcctcaccgg gctcctcttc acagaactgg ctgctgccct ggatgccgaa ggggaaggaa
3301tcagcaaagt acaaaggaaa gctgtcagtg caattcacag cctgctaagt tctcacgacc
3361tggacccacg ctgtgtcaaa ccagaggtga aggtcaaaat cgccgccctt tacctacctt
3421tagttggcat cattttggat gctttgccac agctctgtga ctttacagtt gcagatactc
3481gcagataccg caccagtggc tcggatgaag aacaagaagg agccggtgcc attaaccaga
3541atgtggctct ggccatagca gggaataatt tcaatttgaa aacaagtgga atagtgctgt
3601cttccttgcc ctataagcag tacaacatgc tgaacgcgga cactactcgc aacctcatga
3661tctgcttcct ctggatcatg aaaaatgctg atcagagcct cattaggaag tggattgctg
3721acctgccatc aacgcagctc aacaggattt tagatctact tttcatctgt gtgttatgtt
3781ttgagtataa gggaaaacag agttctgaca aagtcagtac ccaagtcctg cagaagtcaa
3841gggatgtcaa ggcccggctg gaagaggctt tgctccgtgg ggaaggggcc agaggggaga
3901tgatgcgccg ccgggctcca gggaacgacc gatttccagg cctaaatgaa aatttgagat
3961ggaagaaaga gcagacacat tggcggcaag ctaatgagaa gctagataaa acaaaggccg
4021agttagatca agaagccttg atcagtggca atctggctac agaagcacat ttaatcatcc
4081tggatatgca ggaaaacatt atccaggcga gctcggctct ggactgtaaa gacagcctgc
4141tgggaggtgt tctgagggtg ctggtgaatt ctctgaactg tgatcagagt accacctacc
4201tgactcactg ctttgcaaca ctccgtgctc tcatcgccaa gtttggagac ttactcttcg
4261aagaggaggt ggaacagtgt ttcgacctat gtcaccaagt cctgcaccac tgcagcagca
4321gcatggatgt cacccggagc caagcctgtg ccacccttta cctcctcatg aggttcagtt
4381ttggagccac cagtaatttt gcaagagtaa agatgcaagt aaccatgtcc ctggcatctt
4441tggtgggaag agcaccagac tttaatgaag agcacctgag aagatccttg aggacaattt
4501tggcctattc agaagaggac acagccatgc agatgactcc ttttcccacc caggtggagg
4561aacttctctg taatctgaat agcatcttat atgacacagt gaaaatgagg gaatttcagg
4621aagatcctga gatgcttatg gatctcatgt acagaattgc caagagttac caggcatctc
4681ctgatctgcg gctgacctgg ctccagaaca tggcagagaa acacaccaag aagaagtgct
4741acacggaggc tgccatgtgc ctggtgcacg ccgctgcgtt agtggctgag tatctgagca
4801tgctggagga ccacagctac ctgcccgtgg gcagtgtcag cttccagaat atttcttcca
4861atgtgctgga ggagtctgtg gtctctgagg acaccctgtc acctgacgag gatggggtgt
4921gcgcaggcca gtacttcacc gagagtggcc tggtaggcct cctggagcag gccgcggagc
4981tcttcagcac gggaggctta tatgagacag ttaatgaggt ctacaagctg gtcatcccca
5041tcctagaagc gcatcgagaa ttccggaagc tgacactcac tcacagcaag ctgcagagag
5101ccttcgacag catcgttaac aaggatcata agagaatgtt tggaacctac ttccgagttg
5161gtttctttgg atccaaattt ggggatttgg atgaacagga atttgtctac aaagagcctg
5221caattaccaa gcttcctgag atctcacata gactagaggc attttatggt caatgttttg
5281gtgcagaatt tgtggaagtg attaaagact ccactcctgt ggacaaaacc aagttggatc
5341ctaacaaggc ctacatacag atcacttttg tggagcccta ctttgatgag tatgagatga
5401aagacagggt cacatacttt gagaagaatt tcaacctccg gaggttcatg tacaccaccc
5461TGAACAGCCT GGAGGGGCGG CCTCGGGGAG AGCTGCATGA GCAGTACAGA AGGAACACAG
5521TCCTGACCAC TATGCACGCC TTCCCCTTCA TCAAGACCAG GATCAGCGTC ATCCAGAAGG
5581AGGAGTTTGT TTTGACACCG ATTGAAGTTG CCATTGAAGA CATGAAGAAG AAGACCCTGC
5641AGTTAGCAGT TGCCATTAAC CAGGAGCCGC CTGATGCAAA GATGCTTCAG ATGGTGCTGC
5701AAGGCTCTGT GGGAGCTACT GTAAATCAGG GACCACTGGA AGTAGCCCAA GTGTTTTTGG
5761CTGAAATTCC TGCTGATCCA AAACTCTATC GACATCACAA CAAGTTGAGG TTATGCTTTA
5821AGGAATTCAT CATGAGATGT GGTGAAGCTG TAGAGAAAAA CAAGCGTCTC ATCACGGCAG
5881ACCAGAGGGA ATATCAGCAG GAACTCAAAA AGAACTATAA CAAGCTAAAA GAGAACCTCA
5941GGCCAATGAT CGAGCGGAAA ATTCCAGAAC TGTACAAGCC AATATTCAGA GTTGAGAGTC
6001AAAAGAGGGA CTCCTTCCAC AGATCTAGTT TCAGGAAATG TGAAACCCAG TTGTCACAGG
6061GCAGCTTGCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt
6121gttgcaacga ac

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