Construct: ORF BRDN0000395095

Failed QC, DO NOT USE

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016589.1_s300c1
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DIAPH1 (1729)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

No results found.

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3855
ORF length:
3786
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGAGCCGCCC GGCGGGAGAC Aggggcccgg ccgcgggacc cgggacaaga
121agaagggccg gagcccagat gagctgccct cggcgggcgg cgacggcggc aaatctaaga
181aatttctgga gagatttacc agcatgagaa ttaagaagga gaaggaaaag cccaattctg
241ctcatagaaa ttcttctgca tcatatgggg atgatcccac agcacagtca ttgcaagatg
301tttcagatga acaagtgctg gttctctttg aacagatgct gctggatatg aacctgaatg
361aggagaaaca gcaacctttg agggagaagg acatcatcat caagagggag atggtgtccc
421aatacttgta cacctccaag gctggcatga gccagaagga gagctctaag tctgccatga
481tgtatattca ggagttgagg tcaggcttgc gggatatgcc tctgctcagc tgcctggagt
541cccttcgtgt gtctctcaac aacaaccctg tcagttgggt gcaaacattt ggtgctgaag
601gcttggcctc cttattggac attcttaaac gacttcatga tgagaaagaa gagactgctg
661ggagttacga tagccggaac aagcatgaga tcattcgctg cttgaaagct tttatgaaca
721acaagtttgg aatcaagacc atgttggaga cagaagaagg aatcctactg ctggtcagag
781ccatggatcc tgctgttccc aacatgatga ttgatgcagc taagctgctt tctgctcttt
841gtattctacc gcagccagag gacatgaatg aaagggtttt ggaggcaatg acagaaagag
901ctgagatgga tgaagtggaa cgtttccagc cgctgctgga tggattaaaa agtggaacca
961ctattgcact gaaggttgga tgcctacagc tgatcaatgc tctcatcaca ccagcggagg
1021aacttgactt ccgagttcac atcagaagtg aactgatgcg tttggggcta catcaggtgt
1081tgcaggacct tcgagagatt gaaaatgaag atatgagagt gcaactaaat gtgtttgatg
1141aacaagggga agaggattcc tatgacctga agggacggct ggatgacatt cgcatggaga
1201tggatgactt taatgaagtc tttcagattc tcttaaacac agtgaaggat tcaaaggcag
1261agccacactt cctttccatc ctgcagcact tactcttggt ccgaaatgac tatgaggcca
1321gacctcagta ctataagttg attgaagaat gtatttccca gatagttctg cacaagaacg
1381gggctgatcc tgacttcaag tgccggcacc tccagattga gattgaggga ttaattgatc
1441aaatgattga taagacaaag gtggagaaat ctgaagccaa agctgcagag ctggaaaaga
1501agttggactc agagttaaca gcccgacatg agctacaggt ggaaatgaaa aagatggaaa
1561gtgactttga gcagaagctt caagatcttc agggagaaaa agatgcactg cattctgaaa
1621agcagcaaat tgccacagag aaacaggacc tggaagcaga ggtgtcccag ctcacaggag
1681aggttgccaa gctgacaaag gaactggaag atgccaagaa agaaatggct tccctctctg
1741cggcagctat tactgtacct ccttctgttc ctagtcgtgc tcctgttccc cctgcccctc
1801ctttacctgg tgactctggc actattattc caccaccacc tgctcctggg gatagtacca
1861ctcctcctcc tcctcctcct cctcctcctc cacctccttt gcctgggggt gtttgcatct
1921cctcaccccc ttctttacct ggaggtactg ctatctctcc accccctcct ttgtctgggg
1981atgctaccat ccctccaccc cctcctttgc ctgagggtgt tggcatccct tcaccctctt
2041ctttgcctgg aggtactgcc atccccccac ctcctccttt gcctgggagt gctagaatcc
2101ccccaccacc acctcctttg cctgggagtg ctggaattcc ccccccacct cctcccttgc
2161ctggagaagc aggaatgcca cctcctcctc cccctcttcc tggtggtcct ggaatccctc
2221cacctcctcc atttcccgga ggccctggca ttcctccacc tccacccgga atgggtatgc
2281ctccacctcc cccatttgga tttggagttc ctgcagcccc agttctgcca tttggattaa
2341cccccaaaaa gctttataag ccagaggtgc agctccggag gccaaactgg tccaagcttg
2401tggctgagga cctctcccag gactgcttct ggacaaaggt gaaggaggac cgctttgaga
2461acaatgaact tttcgccaaa cttaccctta ccttctctgc ccagaccaag acttccaaag
2521ccaagaagga tcaagaaggt ggagaagaaa agaaatctgt gcaaaagaaa aaagtaaaag
2581agttaaaggt gttggattca aagacagccc agaatctctc aatctttttg ggttccttcc
2641gcatgcccta tcaagagatt aagaatgtca tcctggaggt gaatgaggct gttctgactg
2701agtctatgat ccagaacctc attaagcaaa tgccagagcc agagcagtta aaaatgcttt
2761ctgaactgaa ggatgaatat gatgacctgg ctgagtcaga gcagtttggc gtggtgatgg
2821gcactgtgcc ccgactgcgg cctcgcctca atgccattct cttcaagcta caattcagcg
2881agcaagtgga gaatatcaag ccagagattg tgtctgtcac tgctgcatgt gaggagttac
2941gtaagagtga gagcttttcc aatctcctag agattacctt gcttgttgga aattacatga
3001atgctggctc cagaaatgct ggtgcttttg gcttcaatat cagcttcctc tgtaagcttc
3061gagacaccaa gtccacagat cagaagatga cgttgttaca cttcttggct gagttgtgtg
3121agaatgacta tcccgatgtc ctcaagtttc cagacgagct tgcccatgtg gagaaagcca
3181gccgagtttc tgctgaaaac ttgcaaaaga acctagatca gatgaagaaa caaatttctg
3241atgtggaacg tgatgttcag aatttcccag ctgccacaga tgaaaaagac aagtttgttg
3301aaaaaatgac cagctttgtg aaggatgcac aggaacagta taacaagctg cggatgatgc
3361attctaacat ggagaccctc tataaggagc tgggcgagta cttcctcttt gaccccaaga
3421agttgtctgt tgaagaattt ttcatggatc ttcacaattt tcggaatatg tttttgcaag
3481cagtcaagga gaaccagaCC CGGCGGGAGA CAGAAGAAAA GATGAGGCGA GCAAAACTAG
3541CCAAGGAGAA GGCAGAGAAG GAGCGGCTAG AGAAGCAGCA GAAGAGAGAG CAACTCATAG
3601ACATGAATGC AGAGGGCGAT GAGACAGGTG TGATGGACAG TCTTCTAGAA GCCCTGCAGT
3661CAGGGGCAGC ATTCCGACGG AAGAGAGGGC CCCGTCAAGC CAACAGGAAG GCCGGGTGTG
3721CAGTCACATC TCTGCTAGCT TCGGAGCTGA CCAAGGATGA TGCCATGGCT GCTGTTCCTG
3781CCAAGGTGTC CAAGAACAGT GAGACATTCC CCACAATCCT TGAGGAAGCC AAGGAGTTGG
3841TTGGCCGTGC AAGCTTGCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct
3901tatcaatttg ttgcaacgaa c

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