Construct: ORF BRDN0000395114

Failed QC, DO NOT USE

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006776.1_s300c1
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LRRK2 (120892)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

No results found.

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
7650
ORF length:
7581
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCTAGTGGC AGGTGTCAGT Ggtgcgaaga ggacgaggaa actctgaaga
121agttgatagt caggctgaac aatgtccagg aaggaaaaca gatagaaacg ctggtccaaa
181tcctggagga tctgctggtg ttcacgtact ccgagcacgc ctccaagtta tttcaaggca
241aaaatatcca tgtgcctctg ttgatcgtct tggactccta tatgagagtc gcgagtgtgc
301agcaggtggg ttggtcactt ctgtgcaaat taatagaagt ctgtccaggt acaatgcaaa
361gcttaatggg accccaggat gttggaaatg attgggaagt ccttggtgtt caccaattga
421ttcttaaaat gctaacagtt cataatgcca gtgtaaactt gtcagtgatt ggactgaaga
481ccttagatct cctcctaact tcaggtaaaa tcaccttgct gatactggat gaagaaagtg
541atattttcat gttaattttt gatgccatgc actcatttcc agccaatgat gaagtccaga
601aacttggatg caaagcttta catgtgctgt ttgagagagt ctcagaggag caactgactg
661aatttgttga gaacaaagat tatatgatat tgttaagtgc gttaacaaat tttaaagatg
721aagaggaaat tgtgcttcat gtgctgcatt gtttacattc cctagcgatt ccttgcaata
781atgtggaagt cctcatgagt ggcaatgtca ggtgttataa tattgtggtg gaagctatga
841aagcattccc tatgagtgaa agaattcaag aagtgagttg ctgtttgctc cataggctta
901cattaggtaa ttttttcaat atcctggtat taaacgaagt ccatgagttt gtggtgaaag
961ctgtgcagca gtacccagag aatgcagcat tgcagatctc agcgctcagc tgtttggccc
1021tcctcactga gactattttc ttaaatcaag atttagagga aaagaatgag aatcaagaga
1081atgatgatga gggggaagaa gataaattgt tttggctgga agcctgttac aaagcattaa
1141cgtggcatag aaagaacaag cacgtgcagg aggccgcatg ctgggcacta aataatctcc
1201ttatgtacca aaacagttta catgagaaga ttggagatga agatggccat ttcccagctc
1261atagggaagt gatgctctcc atgctgatgc attcttcatc aaaggaagtt ttccaggcat
1321ctgcgaatgc attgtcaact ctcttagaac aaaatgttaa tttcagaaaa atactgttat
1381caaaaggaat acacctgaat gttttggagt taatgcagaa gcatatacat tctcctgaag
1441tggctgaaag tggctgtaaa atgctaaatc atctttttga aggaagcaac acttccctgg
1501atataatggc agcagtggtc cccaaaatac taacagttat gaaacgtcat gagacatcat
1561taccagtgca gctggaggcg cttcgagcta ttttacattt tatagtgcct ggcatgccag
1621aagaatccag ggaggataca gaatttcatc ataagctaaa tatggttaaa aaacagtgtt
1681tcaagaatga tattcacaaa ctggtcctag cagctttgaa caggttcatt ggaaatcctg
1741ggattcagaa atgtggatta aaagtaattt cttctattgt acattttcct gatgcattag
1801agatgttatc cctggaaggt gctatggatt cagtgcttca cacactgcag atgtatccag
1861atgaccaaga aattcagtgt ctgggtttaa gtcttatagg atacttgatt acaaagaaga
1921atgtgttcat aggaactgga catctgctgg caaaaattct ggtttccagc ttataccgat
1981ttaaggatgt tgctgaaata cagactaaag gatttcagac aatcttagca atcctcaaat
2041tgtcagcatc tttttctaag ctgctggtgc atcattcatt tgacttagta atattccatc
2101aaatgtcttc caatatcatg gaacaaaagg atcaacagtt tctaaacctc tgttgcaagt
2161gttttgcaaa agtagctatg gatgattact taaaaaatgt gatgctagag agagcgtgtg
2221atcagaataa cagcatcatg gttgaatgct tgcttctatt gggagcagat gccaatcaag
2281caaaggaggg atcttcttta atttgtcagg tatgtgagaa agagagcagt cccaaattgg
2341tggaactctt actgaatagt ggatctcgtg aacaagatgt acgaaaagcg ttgacgataa
2401gcattgggaa aggtgacagc cagatcatca gcttgctctt aaggaggctg gccctggatg
2461tggccaacaa tagcatttgc cttggaggat tttgtatagg aaaagttgaa ccttcttggc
2521ttggtccttt atttccagat aagacttcta atttaaggaa acaaacaaat atagcatcta
2581cactagcaag aatggtgatc agatatcaga tgaaaagtgc tgtggaagaa ggaacagcct
2641caggcagcga tggaaatttt tctgaagatg tgctgtctaa atttgatgaa tggaccttta
2701ttcctgactc ttctatggac agtgtgtttg ctcaaagtga tgacctggat agtgaaggaa
2761gtgaaggctc atttcttgtg aaaaagaaat ctaattcaat tagtgtagga gaattttacc
2821gagatgccgt attacagcgt tgctcaccaa atttgcaaag acattccaat tccttggggc
2881ccatttttga tcatgaagat ttactgaagc gaaaaagaaa aatattatct tcagatgatt
2941cactcaggtc atcaaaactt caatcccata tgaggcattc agacagcatt tcttctctgg
3001cttctgagag agaatatatt acatcactag acctttcagc aaatgaacta agagatattg
3061atgccctaag ccagaaatgc tgtataagtg ttcatttgga gcatcttgaa aagctggagc
3121ttcaccagaa tgcactcacg agctttccac aacagctatg tgaaactctg aagagtttga
3181cacatttgga cttgcacagt aataaattta catcatttcc ttcttatttg ttgaaaatga
3241gttgtattgc taatcttgat gtctctcgaa atgacattgg accctcagtg gttttagatc
3301ctacagtgaa atgtccaact ctgaaacagt ttaacctgtc atataaccag ctgtcttttg
3361tacctgagaa cctcactgat gtggtagaga aactggagca gctcatttta gaaggaaata
3421aaatatcagg gatatgctcc cccttgagac tgaaggaact gaagatttta aaccttagta
3481agaaccacat ttcatcccta tcagagaact ttcttgaggc ttgtcctaaa gtggagagtt
3541tcagtgccag aatgaatttt cttgctgcta tgcctttctt gcctccttct atgacaatcc
3601taaaattatc tcagaacaaa ttttcctgta ttccagaagc aattttaaat cttccacact
3661tgcggtcttt agatatgagc agcaatgata ttcagtacct accaggtccc gcacactgga
3721aatctttgaa cttaagggaa ctcttattta gccataatca gatcagcatc ttggacttga
3781gtgaaaaagc atatttatgg tctagagtag agaaactgca tctttctcac aataaactga
3841aagagattcc tcctgagatt ggctgtcttg aaaatctgac atctctggat gtcagttaca
3901acttggaact aagatccttt cccaatgaaa tggggaaatt aagcaaaata tgggatcttc
3961ctttggatga actgcatctt aactttgatt ttaaacatat aggatgtaaa gccaaagaca
4021tcataaggtt tcttcaacag cgattaaaaa aggctgtgcc ttataaccga atgaaactta
4081tgattgtggg aaatactggg agtggtaaaa ccaccttatt gcagcaatta atgaaaacca
4141agaaatcaga tcttggaatg caaagtgcca cagttggcat agatgtgaaa gactggccta
4201tccaaataag agacaaaaga aagagagatc tcgtcctaaa tgtgtgggat tttgcaggtc
4261gtgaggaatt ctatagtact catccccatt ttatgacgca gcgagcattg taccttgctg
4321tctatgacct cagcaaggga caggctgaag ttgatgccat gaagccttgg ctcttcaata
4381taaaggctcg cgcttcttct tcccctgtga ttctcgttgg cacacatttg gatgtttctg
4441atgagaagca acgcaaagcc tgcatgagta aaatcaccaa ggaactcctg aataagcgag
4501ggttccctgc catacgagat taccactttg tgaatgccac cgaggaatct gatgctttgg
4561caaaacttcg gaaaaccatc ataaacgaga gccttaattt caagatccga gatcagcttg
4621ttgttggaca gctgattcca gactgctatg tagaacttga aaaaatcatt ttatcggagc
4681gtaaaaatgt gccaattgaa tttcccgtaa ttgaccggaa acgattatta caactagtga
4741gagaaaatca gctgcagtta gatgaaaatg agcttcctca cgcagttcac tttctaaatg
4801aatcaggagt ccttcttcat tttcaagacc cagcactgca gttaagtgac ttgtactttg
4861tggaacccaa gtggctttgt aaaatcatgg cacagatttt gacagtgaaa gtggaaggtt
4921gtccaaaaca ccctaaggga attatttcgc gtagagatgt ggaaaaattt ctttcaaaga
4981aaaggaaatt tccaaagaac tacatgacac agtattttaa gctcctagaa aaattccaga
5041ttgctttgcc aataggagaa gaatatttgc tggttccaag cagtttgtct gaccacaggc
5101ctgtgataga gcttccccat tgtgagaact ctgaaattat catccgacta tatgaaatgc
5161cttattttcc aatgggattt tggtcaagat taatcaatcg attacttgag atttcacctt
5221acatgctttc agggagagaa cgagcacttc gcccaaacag aatgtattgg cgacaaggca
5281tttacttaaa ttggtctcct gaagcttatt gtctggtagg atctgaagtc ttagacaatc
5341atccagagag tttcttaaaa attacagttc cttcttgtag aaaaggctgt attcttttgg
5401gccaagttgt ggaccacatt gattctctca tggaagaatg gtttcctggg ttgctggaga
5461ttgatatttg tggtgaagga gaaactctgt tgaagaaatg ggcattatat agttttaatg
5521atggcgaaga acatcaaaaa atcttacttg atgacttgat gaagaaagca gaggaaggag
5581atctcttagt aaatccagat caaccaaggc tcaccattcc aatatctcag attgcccctg
5641acttgatttt ggctgacctg cctagaaata ttatgttgaa taatgatgag ttggaatttg
5701aacaagctcc agagtttctc ctaggtgatg gcagttttgg atcagtttac cgagcagcct
5761atgaaggaga agaagtggct gtgaagattt ttaataaaca tacatcactc aggctgttaa
5821gacaagagct tgtggtgctt tgccacctcc accaccccag tttgatatct ttgctggcag
5881ctgggattcg tccccggatg ttggtgatgg agttagcctc caagggttcc ttggatcgcc
5941tgcttcagca ggacaaagcc agcctcacta gaaccctaca gcacaggatt gcactccacg
6001tagctgatgg tttgagatac ctccactcag ccatgattat ataccgagac ctgaaacccc
6061acaatgtgct gcttttcaca ctgtatccca atgctgccat cattgcaaag attgctgact
6121acggcattgc tcagtactgc tgtagaatgg ggataaaaac atcagagggc acaccagggt
6181ttcgtgcacc tgaagttgcc agaggaaatg tcatttataa ccaacaggct gatgtttatt
6241catttggttt actactctat gacattttga caactggagg tagaatagta gagggtttga
6301agtttccaaa tgagtttgat gaattagaaa tacaaggaaa attacctgat ccagttaaag
6361aatatggttg tgccccatgg cctatggttg aaaaattaat taaacagtgt ttgaaagaaa
6421atcctcaaga aaggcctact tctgcccagg tctttgacat tttgaattca gctgaattag
6481tctgtctgac gagacgcatt ttattaccta aaaacgtaat tgttgaatgc atggttgcta
6541cacatcacaa cagcaggaat gcaagcattt ggctgggctg tgggcacacc gacagaggac
6601agctctcatt tcttgactta aatactgaag gatacacttc tgaggaagtt gctgatagta
6661gaatattgtg cttagccttg gtgcatcttc ctgttgaaaa ggaaagctgg attgtgtctg
6721ggacacagtc tggtactctc ctggtcatca ataccgaaga tgggaaaaag agacataccc
6781tagaaaagat gactgattct gtcacttgtt tgtattgcaa ttccttttcc aagcaaagca
6841aacaaaaaaa ttttcttttg gttggaaccg ctgatggcaa gttagcaatt tttgaagata
6901agactgttaa gcttaaagga gctgctcctt tgaagatact aaatatagga aatgtcagta
6961ctccattgat gtgtttgagt gaatccacaa attcaacgga aagaaatgta atgtggggag
7021gatgtggcac aaagattttc tccttttcta atgatttcac cattcagaaa ctcattgaga
7081caagaacaag ccaactgttt tcttatGCAG CTGTCAGTGA TTCCAACATC ATAACAGTGG
7141TGGTAGACAC TGCTCTCTAT ATTGCTAAGC AAAATAGCCC TGTTGTGGAA GTGTGGGATA
7201AGAAAACTGA AAAACTCTGT GGACTAATAG ACTGCGTGCA CTTTTTAAGG GAGGTAACGG
7261TAAAAGAAAA CAAGGAATCA AAACACAAAA TGTCTTATTC TGGGAGAGTG AAAACCCTCT
7321GCCTTCAGAA GAACACTTCT CTTTGGATAG GAACTGGAGG AGGCCATATT TTACTCCTGG
7381ATCTTTCAAC TCGTCGACTT ATACGTGTAA TTTACAACTT TTGTAATTCG GTCAGAGTCA
7441TGATGACAGC ACAGCTAGGA AGCCTTAAAA ATGTCATGCT GGTATTGGGC TACAACCGGA
7501AAAATACTGA AGGTACACAA AAGCAGAAAG AGATACAATC TTGCTTGACC GTTTGGGACA
7561TCAATCTTCC ACATGAAGTG CAAAATTTAG AAAAACACAT TGAAGTGAGA AAAGAATTAG
7621CTGAAAAAAT GAGACGAACA TCTGTTGAGT TGCCAACTTT CTTGTACAAA GTtggcatta
7681taagaaagca ttgcttatca atttgttgca acgaac

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