Construct: ORF BRDN0000464778
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORFC00002.1_s302c1, pLX302-HcRed
- Derived from:
- BRDN0000464765
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HcRed (HcRed)
Vector Information:
- Vector Backbone:
- pLX_302
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- CMV-BRDN0000464778
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | CONTROL | HcRed | HcRed | Hahn Lab HcRed | HcRed.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 753
- ORF length:
- 684
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgagcggc ctgctgaagg agagtatgcg catcaagatg tacatggagg 121 gcaccgtgaa cggccactac ttcaagtgcg agggcgaggg cgacggcaac cccttcgccg 181 gcacccagag catgagaatc cacgtgaccg agggcgcccc cctgcccttc gccttcgaca 241 tcctggcccc ctgctgcgag tacggcagca ggaccttcgt gcaccacacc gccgagatcc 301 ccgacttctt caagcagagc ttccccgagg gcttcacctg ggagagaacc accacctacg 361 aggacggcgg catcctgacc gcccaccagg acaccagcct ggagggcaac tgcctgatct 421 acaaggtgaa ggtgcacggc accaacttcc ccgccgacgg ccccgtgatg aagaacaaga 481 gcggcggctg ggagcccagc accgaggtgg tgtaccccga gaacggcgtg ctgtgcggcc 541 ggaacgtgat ggccctgaag gtgggcgacc ggcacctgat ctgccaccac tacaccagct 601 accggagcaa gaaggccgtg cgcgccctga ccatgcccgg cttccacttc accgacatcc 661 ggctccagat gctgcggaag aagaaggacg agtacttcga gctgtacgag gccagcgtgg 721 cccggtacag cgacctgccc gagaaggcca acttgccaac tttcttgtac aaagtggttg 781 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 841 accggtggcg cgttaagtcg acaatcaacc tctggatta