Construct: shRNA TRCN0000164378

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016534.2-1740s1c1
DNA Barcode:
CCTGAAGGATTAGATGTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPKAPK5-AS1 (51275)
Gene Description:
MAPKAPK5 antisense RNA 1
Transcript:
RefSeq NM_016534.2 (NON-CURRENT)
Match location:
Position 1740 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000164378)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGGATTAGATGTGGAA
Hairpin Sequence:
5'-CCGG-CCTGAAGGATTAGATGTGGAA-CTCGAG-TTCCACATCTAATCCTTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGGATTAGATGTGGAACTCGAGTTCCACATCTAATCCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGGATTAGATGTGGAACTCGAGTTCCACATCTAATCCTTCAGG-3'

Other clones with same target sequence:

(none)