Construct: shRNA TRCN0000217955

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011878.2-3114s21c1
DNA Barcode:
AGCATCTCAACCGGATATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tiam2 (24001)
Gene Description:
T cell lymphoma invasion and metastasis 2
Transcript:
RefSeq NM_011878.2 (CURRENT)
Match location:
Position 3114 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217955)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCATCTCAACCGGATATTTA
Hairpin Sequence:
5'-CCGG-AGCATCTCAACCGGATATTTA-CTCGAG-TAAATATCCGGTTGAGATGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCATCTCAACCGGATATTTACTCGAGTAAATATCCGGTTGAGATGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCATCTCAACCGGATATTTACTCGAGTAAATATCCGGTTGAGATGCT-3'

Other clones with same target sequence:

(none)