Construct: shRNA TRCN0000217998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001744.3-769s21c1
DNA Barcode:
CATGTGGTCTGTAGGAATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK4 (814)
Gene Description:
calcium/calmodulin dependent protein kinase IV
Transcript:
RefSeq NM_001744.3 (NON-CURRENT)
Match location:
Position 769 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGTGGTCTGTAGGAATAAT
Hairpin Sequence:
5'-CCGG-CATGTGGTCTGTAGGAATAAT-CTCGAG-ATTATTCCTACAGACCACATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGTGGTCTGTAGGAATAATCTCGAGATTATTCCTACAGACCACATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGTGGTCTGTAGGAATAATCTCGAGATTATTCCTACAGACCACATG-3'

Other clones with same target sequence:

(none)