Construct: shRNA TRCN0000218022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007125.3-2001s21c1
DNA Barcode:
GGATGCTTTACAGGCATATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UTY (7404)
Gene Description:
ubiquitously transcribed tetratricopeptide repeat containing, Y-linked
Transcript:
RefSeq NM_007125.3 (NON-CURRENT)
Match location:
Position 2001 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATGCTTTACAGGCATATAT
Hairpin Sequence:
5'-CCGG-GGATGCTTTACAGGCATATAT-CTCGAG-ATATATGCCTGTAAAGCATCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATGCTTTACAGGCATATATCTCGAGATATATGCCTGTAAAGCATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATGCTTTACAGGCATATATCTCGAGATATATGCCTGTAAAGCATCC-3'

Other clones with same target sequence:

(none)