Construct: shRNA TRCN0000218031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182663.2-1186s21c1
DNA Barcode:
CTTCAGAACTTCCTAACAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RASSF5 (83593)
Gene Description:
Ras association domain family member 5
Transcript:
RefSeq NM_182663.2 (NON-CURRENT)
Match location:
Position 1186 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAGAACTTCCTAACAATC
Hairpin Sequence:
5'-CCGG-CTTCAGAACTTCCTAACAATC-CTCGAG-GATTGTTAGGAAGTTCTGAAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAGAACTTCCTAACAATCCTCGAGGATTGTTAGGAAGTTCTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAGAACTTCCTAACAATCCTCGAGGATTGTTAGGAAGTTCTGAAG-3'

Other clones with same target sequence:

(none)