Construct: shRNA TRCN0000218037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081035.1-6053s21c1
DNA Barcode:
CAATTACCCAAAGGTACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nav3 (260315)
Gene Description:
neuron navigator 3
Transcript:
RefSeq NM_001081035.1 (CURRENT)
Match location:
Position 6053 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATTACCCAAAGGTACTTTA
Hairpin Sequence:
5'-CCGG-CAATTACCCAAAGGTACTTTA-CTCGAG-TAAAGTACCTTTGGGTAATTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATTACCCAAAGGTACTTTACTCGAGTAAAGTACCTTTGGGTAATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATTACCCAAAGGTACTTTACTCGAGTAAAGTACCTTTGGGTAATTG-3'

Other clones with same target sequence:

  1. TRCN0000303440