Construct: shRNA TRCN0000218058

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022464.4-633s21c1
DNA Barcode:
ATGAGCTGAATGTTGTCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIL1 (64374)
Gene Description:
SIL1 nucleotide exchange factor
Transcript:
RefSeq NM_022464.4 (NON-CURRENT)
Match location:
Position 633 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218058)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAGCTGAATGTTGTCATTG
Hairpin Sequence:
5'-CCGG-ATGAGCTGAATGTTGTCATTG-CTCGAG-CAATGACAACATTCAGCTCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAGCTGAATGTTGTCATTGCTCGAGCAATGACAACATTCAGCTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAGCTGAATGTTGTCATTGCTCGAGCAATGACAACATTCAGCTCAT-3'

Other clones with same target sequence:

(none)