Construct: shRNA TRCN0000218128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033115.2-1602s21c1
DNA Barcode:
CAAGTACGATGCAATTGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBCK (93627)
Gene Description:
TBC1 domain containing kinase
Transcript:
RefSeq NM_033115.2 (NON-CURRENT)
Match location:
Position 1602 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGTACGATGCAATTGATAA
Hairpin Sequence:
5'-CCGG-CAAGTACGATGCAATTGATAA-CTCGAG-TTATCAATTGCATCGTACTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGTACGATGCAATTGATAACTCGAGTTATCAATTGCATCGTACTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGTACGATGCAATTGATAACTCGAGTTATCAATTGCATCGTACTTG-3'

Other clones with same target sequence:

(none)