Construct: shRNA TRCN0000218171

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001105522.1-1117s21c1
DNA Barcode:
ACTTTCACCTCTGGCAAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM49D2 (729384)
Gene Description:
tripartite motif containing 49D2
Transcript:
RefSeq NM_001105522.1 (CURRENT)
Match location:
Position 1117 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218171)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTTCACCTCTGGCAAATAT
Hairpin Sequence:
5'-CCGG-ACTTTCACCTCTGGCAAATAT-CTCGAG-ATATTTGCCAGAGGTGAAAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTTCACCTCTGGCAAATATCTCGAGATATTTGCCAGAGGTGAAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTTCACCTCTGGCAAATATCTCGAGATATTTGCCAGAGGTGAAAGT-3'

Other clones with same target sequence:

(none)