Construct: shRNA TRCN0000218221

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005604.2-1141s21c1
DNA Barcode:
TGAAGCCTTTGTTGAACAAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POU3F2 (5454)
Gene Description:
POU class 3 homeobox 2
Transcript:
RefSeq NM_005604.2 (NON-CURRENT)
Match location:
Position 1141 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218221)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGCCTTTGTTGAACAAGT
Hairpin Sequence:
5'-CCGG-TGAAGCCTTTGTTGAACAAGT-CTCGAG-ACTTGTTCAACAAAGGCTTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGCCTTTGTTGAACAAGTCTCGAGACTTGTTCAACAAAGGCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGCCTTTGTTGAACAAGTCTCGAGACTTGTTCAACAAAGGCTTCA-3'

Other clones with same target sequence:

(none)