Construct: shRNA TRCN0000218264

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173019.5-1458s21c1
DNA Barcode:
ACCTCAGAATGTAGACATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pfkfb4 (270198)
Gene Description:
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
Transcript:
RefSeq NM_173019.5 (CURRENT)
Match location:
Position 1458 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218264)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTCAGAATGTAGACATATC
Hairpin Sequence:
5'-CCGG-ACCTCAGAATGTAGACATATC-CTCGAG-GATATGTCTACATTCTGAGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTCAGAATGTAGACATATCCTCGAGGATATGTCTACATTCTGAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTCAGAATGTAGACATATCCTCGAGGATATGTCTACATTCTGAGGT-3'

Other clones with same target sequence:

(none)