Construct: shRNA TRCN0000218269
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_913893.3-3629s21c1
- DNA Barcode:
- TAACATGTTACCACGTGTTTC
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC638024 (638024)
- Gene Description:
- similar to AF4/FMR2 family member 3 (LAF-4 protein) (Lymphoid nuclear protein related to AF4)
- Transcript:
- RefSeq XM_913893.3 (NON-CURRENT)
- Match location:
- Position 3629 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000218269)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TAACATGTTACCACGTGTTTC
- Hairpin Sequence:
-
5'-CCGG-TAACATGTTACCACGTGTTTC-CTCGAG-GAAACACGTGGTAACATGTTA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTAACATGTTACCACGTGTTTCCTCGAGGAAACACGTGGTAACATGTTATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATAACATGTTACCACGTGTTTCCTCGAGGAAACACGTGGTAACATGTTA-3'
Other clones with same target sequence:
(none)
