Construct: shRNA TRCN0000218281

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_987077.1-347s21c1
DNA Barcode:
ATGAAGGGAAACTGTCGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prune2 (77754)
Gene Description:
prune homolog 2 (Drosophila)
Transcript:
RefSeq XM_987077.1 (NON-CURRENT)
Match location:
Position 347 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218281)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAAGGGAAACTGTCGATAA
Hairpin Sequence:
5'-CCGG-ATGAAGGGAAACTGTCGATAA-CTCGAG-TTATCGACAGTTTCCCTTCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAAGGGAAACTGTCGATAACTCGAGTTATCGACAGTTTCCCTTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAAGGGAAACTGTCGATAACTCGAGTTATCGACAGTTTCCCTTCAT-3'

Other clones with same target sequence:

(none)