Construct: shRNA TRCN0000218322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002838.3-3118s21c1
DNA Barcode:
TATAACAGAGTGCCACTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRC (5788)
Gene Description:
protein tyrosine phosphatase receptor type C
Transcript:
RefSeq NM_002838.3 (NON-CURRENT)
Match location:
Position 3118 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATAACAGAGTGCCACTTAAA
Hairpin Sequence:
5'-CCGG-TATAACAGAGTGCCACTTAAA-CTCGAG-TTTAAGTGGCACTCTGTTATA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATAACAGAGTGCCACTTAAACTCGAGTTTAAGTGGCACTCTGTTATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATAACAGAGTGCCACTTAAACTCGAGTTTAAGTGGCACTCTGTTATA-3'

Other clones with same target sequence:

(none)