Construct: shRNA TRCN0000218331

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014611.1-16918s21c1
DNA Barcode:
AGAGTCCAAAGTGAGACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MDN1 (23195)
Gene Description:
midasin AAA ATPase 1
Transcript:
RefSeq NM_014611.1 (NON-CURRENT)
Match location:
Position 16918 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218331)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGTCCAAAGTGAGACTTAA
Hairpin Sequence:
5'-CCGG-AGAGTCCAAAGTGAGACTTAA-CTCGAG-TTAAGTCTCACTTTGGACTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGTCCAAAGTGAGACTTAACTCGAGTTAAGTCTCACTTTGGACTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGTCCAAAGTGAGACTTAACTCGAGTTAAGTCTCACTTTGGACTCT-3'

Other clones with same target sequence:

(none)