Construct: shRNA TRCN0000218379

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026792.3-2173s21c1
DNA Barcode:
TGAGCCGTTAAGTACTTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Agpat5 (52123)
Gene Description:
1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon)
Transcript:
RefSeq NM_026792.3 (CURRENT)
Match location:
Position 2173 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218379)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAGCCGTTAAGTACTTATAA
Hairpin Sequence:
5'-CCGG-TGAGCCGTTAAGTACTTATAA-CTCGAG-TTATAAGTACTTAACGGCTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAGCCGTTAAGTACTTATAACTCGAGTTATAAGTACTTAACGGCTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAGCCGTTAAGTACTTATAACTCGAGTTATAAGTACTTAACGGCTCA-3'

Other clones with same target sequence:

(none)