Construct: shRNA TRCN0000218385
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_893233.1-274s21c1
- DNA Barcode:
- TTGACACTCTTCATCAGTTTC
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gprc2a-rs4 (12378)
- Gene Description:
- G protein-coupled receptor, family C, group 2, member A, related sequence 4
- Transcript:
- RefSeq XM_893233.1 (NON-CURRENT)
- Match location:
- Position 274 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000218385)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TTGACACTCTTCATCAGTTTC
- Hairpin Sequence:
-
5'-CCGG-TTGACACTCTTCATCAGTTTC-CTCGAG-GAAACTGATGAAGAGTGTCAA-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTTGACACTCTTCATCAGTTTCCTCGAGGAAACTGATGAAGAGTGTCAATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATTGACACTCTTCATCAGTTTCCTCGAGGAAACTGATGAAGAGTGTCAA-3'
Other clones with same target sequence:
(none)
