Construct: shRNA TRCN0000218427

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005815.4-1612s21c1
DNA Barcode:
ACTGGAGAGAAACCCTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF443 (10224)
Gene Description:
zinc finger protein 443
Transcript:
RefSeq NM_005815.4 (NON-CURRENT)
Match location:
Position 1612 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218427)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGAGAGAAACCCTATAAA
Hairpin Sequence:
5'-CCGG-ACTGGAGAGAAACCCTATAAA-CTCGAG-TTTATAGGGTTTCTCTCCAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGAGAGAAACCCTATAAACTCGAGTTTATAGGGTTTCTCTCCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGAGAGAAACCCTATAAACTCGAGTTTATAGGGTTTCTCTCCAGT-3'

Other clones with same target sequence:

  1. TRCN0000374174