Construct: shRNA TRCN0000218427
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005815.4-1612s21c1
- DNA Barcode:
- ACTGGAGAGAAACCCTATAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ZNF443 (10224)
- Gene Description:
- zinc finger protein 443
- Transcript:
- RefSeq NM_005815.4 (NON-CURRENT)
- Match location:
- Position 1612 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000218427)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ACTGGAGAGAAACCCTATAAA
- Hairpin Sequence:
-
5'-CCGG-ACTGGAGAGAAACCCTATAAA-CTCGAG-TTTATAGGGTTTCTCTCCAGT-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGACTGGAGAGAAACCCTATAAACTCGAGTTTATAGGGTTTCTCTCCAGTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAACTGGAGAGAAACCCTATAAACTCGAGTTTATAGGGTTTCTCTCCAGT-3'
Other clones with same target sequence:
-
TRCN0000374174
