Construct: shRNA TRCN0000218464

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009281.3-1132s21c1
DNA Barcode:
ACAGGAGAAAGACCTTATTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp143 (20841)
Gene Description:
zinc finger protein 143
Transcript:
RefSeq NM_009281.3 (CURRENT)
Match location:
Position 1132 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218464)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGGAGAAAGACCTTATTAC
Hairpin Sequence:
5'-CCGG-ACAGGAGAAAGACCTTATTAC-CTCGAG-GTAATAAGGTCTTTCTCCTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGGAGAAAGACCTTATTACCTCGAGGTAATAAGGTCTTTCTCCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGGAGAAAGACCTTATTACCTCGAGGTAATAAGGTCTTTCTCCTGT-3'

Other clones with same target sequence:

(none)