Construct: shRNA TRCN0000218576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020583.5-411s21c1
DNA Barcode:
AGTCCTGTATGACAAGTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Isg20 (57444)
Gene Description:
interferon-stimulated protein
Transcript:
RefSeq NM_020583.5 (CURRENT)
Match location:
Position 411 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCCTGTATGACAAGTACAT
Hairpin Sequence:
5'-CCGG-AGTCCTGTATGACAAGTACAT-CTCGAG-ATGTACTTGTCATACAGGACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCCTGTATGACAAGTACATCTCGAGATGTACTTGTCATACAGGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCCTGTATGACAAGTACATCTCGAGATGTACTTGTCATACAGGACT-3'

Other clones with same target sequence:

(none)