Construct: shRNA TRCN0000218587

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_985579.1-725s21c1
DNA Barcode:
GAGTAGTCTCCAGTATCATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
EG666715 (666715)
Gene Description:
predicted gene, EG666715
Transcript:
RefSeq XM_985579.1 (NON-CURRENT)
Match location:
Position 725 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218587)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTAGTCTCCAGTATCATAA
Hairpin Sequence:
5'-CCGG-GAGTAGTCTCCAGTATCATAA-CTCGAG-TTATGATACTGGAGACTACTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTAGTCTCCAGTATCATAACTCGAGTTATGATACTGGAGACTACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTAGTCTCCAGTATCATAACTCGAGTTATGATACTGGAGACTACTC-3'

Other clones with same target sequence:

(none)