Construct: shRNA TRCN0000218594

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005816.4-4254s21c1
DNA Barcode:
ACATAGCGACAGATAACTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CD96 (10225)
Gene Description:
CD96 molecule
Transcript:
RefSeq NM_005816.4 (NON-CURRENT)
Match location:
Position 4254 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218594)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATAGCGACAGATAACTATA
Hairpin Sequence:
5'-CCGG-ACATAGCGACAGATAACTATA-CTCGAG-TATAGTTATCTGTCGCTATGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATAGCGACAGATAACTATACTCGAGTATAGTTATCTGTCGCTATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATAGCGACAGATAACTATACTCGAGTATAGTTATCTGTCGCTATGT-3'

Other clones with same target sequence:

(none)