Construct: shRNA TRCN0000218597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014011.4-1109s21c1
DNA Barcode:
TGCAATTCCACAAGCTAATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SOCS5 (9655)
Gene Description:
suppressor of cytokine signaling 5
Transcript:
RefSeq NM_014011.4 (CURRENT)
Match location:
Position 1109 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAATTCCACAAGCTAATTG
Hairpin Sequence:
5'-CCGG-TGCAATTCCACAAGCTAATTG-CTCGAG-CAATTAGCTTGTGGAATTGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAATTCCACAAGCTAATTGCTCGAGCAATTAGCTTGTGGAATTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAATTCCACAAGCTAATTGCTCGAGCAATTAGCTTGTGGAATTGCA-3'

Other clones with same target sequence:

(none)