Construct: shRNA TRCN0000218609

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001048054.1-931s21c1
DNA Barcode:
CAAAGCATAAGGTTGACATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dusp16 (70686)
Gene Description:
dual specificity phosphatase 16
Transcript:
RefSeq NM_001048054.1 (NON-CURRENT)
Match location:
Position 931 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218609)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGCATAAGGTTGACATTG
Hairpin Sequence:
5'-CCGG-CAAAGCATAAGGTTGACATTG-CTCGAG-CAATGTCAACCTTATGCTTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGCATAAGGTTGACATTGCTCGAGCAATGTCAACCTTATGCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGCATAAGGTTGACATTGCTCGAGCAATGTCAACCTTATGCTTTG-3'

Other clones with same target sequence:

(none)