Construct: shRNA TRCN0000218678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173826.3-1610s21c1
DNA Barcode:
GCAAATCTCCAGTGGTTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCAIM (285343)
Gene Description:
T cell activation inhibitor, mitochondrial
Transcript:
RefSeq NM_173826.3 (NON-CURRENT)
Match location:
Position 1610 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAATCTCCAGTGGTTTATT
Hairpin Sequence:
5'-CCGG-GCAAATCTCCAGTGGTTTATT-CTCGAG-AATAAACCACTGGAGATTTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAATCTCCAGTGGTTTATTCTCGAGAATAAACCACTGGAGATTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAATCTCCAGTGGTTTATTCTCGAGAATAAACCACTGGAGATTTGC-3'

Other clones with same target sequence:

(none)