Construct: shRNA TRCN0000218800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006802.2-2067s21c1
DNA Barcode:
TAGTTAGCCATTGATGCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SF3A3 (10946)
Gene Description:
splicing factor 3a subunit 3
Transcript:
RefSeq NM_006802.2 (NON-CURRENT)
Match location:
Position 2067 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGTTAGCCATTGATGCAAAT
Hairpin Sequence:
5'-CCGG-TAGTTAGCCATTGATGCAAAT-CTCGAG-ATTTGCATCAATGGCTAACTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGTTAGCCATTGATGCAAATCTCGAGATTTGCATCAATGGCTAACTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGTTAGCCATTGATGCAAATCTCGAGATTTGCATCAATGGCTAACTA-3'

Other clones with same target sequence:

(none)