Construct: shRNA TRCN0000218826

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020856.2-4107s21c1
DNA Barcode:
AGATACCACAGATAGCATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSHZ3 (57616)
Gene Description:
teashirt zinc finger homeobox 3
Transcript:
RefSeq NM_020856.2 (NON-CURRENT)
Match location:
Position 4107 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218826)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATACCACAGATAGCATTTA
Hairpin Sequence:
5'-CCGG-AGATACCACAGATAGCATTTA-CTCGAG-TAAATGCTATCTGTGGTATCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATACCACAGATAGCATTTACTCGAGTAAATGCTATCTGTGGTATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATACCACAGATAGCATTTACTCGAGTAAATGCTATCTGTGGTATCT-3'

Other clones with same target sequence:

(none)