Construct: shRNA TRCN0000218918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022748.10-1235s21c1
DNA Barcode:
GACTATGGGAAGGTTGAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNS3 (64759)
Gene Description:
tensin 3
Transcript:
RefSeq NM_022748.10 (NON-CURRENT)
Match location:
Position 1235 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTATGGGAAGGTTGAATTA
Hairpin Sequence:
5'-CCGG-GACTATGGGAAGGTTGAATTA-CTCGAG-TAATTCAACCTTCCCATAGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTATGGGAAGGTTGAATTACTCGAGTAATTCAACCTTCCCATAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTATGGGAAGGTTGAATTACTCGAGTAATTCAACCTTCCCATAGTC-3'

Other clones with same target sequence:

(none)