Construct: shRNA TRCN0000218924

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001132080.2-2506s21c1
DNA Barcode:
GGCTACCTTAGTGGAAATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK4P3 (645619)
Gene Description:
adenylate kinase 4 pseudogene 3
Transcript:
RefSeq XM_001132080.2 (NON-CURRENT)
Match location:
Position 2506 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218924)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTACCTTAGTGGAAATATA
Hairpin Sequence:
5'-CCGG-GGCTACCTTAGTGGAAATATA-CTCGAG-TATATTTCCACTAAGGTAGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTACCTTAGTGGAAATATACTCGAGTATATTTCCACTAAGGTAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTACCTTAGTGGAAATATACTCGAGTATATTTCCACTAAGGTAGCC-3'

Other clones with same target sequence:

(none)