Construct: shRNA TRCN0000218938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021455.3-2022s21c1
DNA Barcode:
AGAAGAGGCGGTTCAATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mlxipl (58805)
Gene Description:
MLX interacting protein-like
Transcript:
RefSeq NM_021455.3 (NON-CURRENT)
Match location:
Position 2022 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGAGGCGGTTCAATATTA
Hairpin Sequence:
5'-CCGG-AGAAGAGGCGGTTCAATATTA-CTCGAG-TAATATTGAACCGCCTCTTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGAGGCGGTTCAATATTACTCGAGTAATATTGAACCGCCTCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGAGGCGGTTCAATATTACTCGAGTAATATTGAACCGCCTCTTCT-3'

Other clones with same target sequence:

(none)