Construct: shRNA TRCN0000218970

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001003850.1-1298s21c1
DNA Barcode:
TGAAGATTGCATGGTGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gon4l (76022)
Gene Description:
gon-4-like (C.elegans)
Transcript:
RefSeq XM_001003850.1 (NON-CURRENT)
Match location:
Position 1298 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218970)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGATTGCATGGTGGAAAT
Hairpin Sequence:
5'-CCGG-TGAAGATTGCATGGTGGAAAT-CTCGAG-ATTTCCACCATGCAATCTTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGATTGCATGGTGGAAATCTCGAGATTTCCACCATGCAATCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGATTGCATGGTGGAAATCTCGAGATTTCCACCATGCAATCTTCA-3'

Other clones with same target sequence:

(none)