Construct: shRNA TRCN0000218991

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_148413.2-2789s21c1
DNA Barcode:
GATACTCCTTGATGGATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myo3a (667663)
Gene Description:
myosin IIIA
Transcript:
RefSeq NM_148413.2 (NON-CURRENT)
Match location:
Position 2789 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000218991)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATACTCCTTGATGGATTTAT
Hairpin Sequence:
5'-CCGG-GATACTCCTTGATGGATTTAT-CTCGAG-ATAAATCCATCAAGGAGTATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATACTCCTTGATGGATTTATCTCGAGATAAATCCATCAAGGAGTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATACTCCTTGATGGATTTATCTCGAGATAAATCCATCAAGGAGTATC-3'

Other clones with same target sequence:

(none)