Construct: shRNA TRCN0000219055

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019995.3-1601s21c1
DNA Barcode:
ATAGTTCCTCAGGTAACTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Supt20 (56790)
Gene Description:
suppressor of Ty 20
Transcript:
RefSeq NM_019995.3 (CURRENT)
Match location:
Position 1601 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219055)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAGTTCCTCAGGTAACTATT
Hairpin Sequence:
5'-CCGG-ATAGTTCCTCAGGTAACTATT-CTCGAG-AATAGTTACCTGAGGAACTAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAGTTCCTCAGGTAACTATTCTCGAGAATAGTTACCTGAGGAACTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAGTTCCTCAGGTAACTATTCTCGAGAATAGTTACCTGAGGAACTAT-3'

Other clones with same target sequence:

(none)