Construct: shRNA TRCN0000219059

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139126.2-1864s21c1
DNA Barcode:
ATACAGATGTTGTCGACATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPIL4 (85313)
Gene Description:
peptidylprolyl isomerase like 4
Transcript:
RefSeq NM_139126.2 (NON-CURRENT)
Match location:
Position 1864 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219059)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATACAGATGTTGTCGACATTT
Hairpin Sequence:
5'-CCGG-ATACAGATGTTGTCGACATTT-CTCGAG-AAATGTCGACAACATCTGTAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATACAGATGTTGTCGACATTTCTCGAGAAATGTCGACAACATCTGTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATACAGATGTTGTCGACATTTCTCGAGAAATGTCGACAACATCTGTAT-3'

Other clones with same target sequence:

(none)